Overview

  • Product name
  • Description
    Rabbit polyclonal to CD2AP
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A peptide from the C terminal residues of human CD2AP.

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab84829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500.

Target

  • Function
    Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
  • Tissue specificity
    Widely expressed in fetal and adult tissues.
  • Involvement in disease
    Defects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Contains 3 SH3 domains.
  • Domain
    The Pro-rich domain may mediate binding to SH3 domains.
    Potential homodimerization is mediated by the coiled coil domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter protein CMS antibody
    • AL024079 antibody
    • C78928 antibody
    • Cas ligand with multiple SH3 domains antibody
    • CD2 associated protein antibody
    • CD2-associated protein antibody
    • CD2AP antibody
    • CD2AP_HUMAN antibody
    • CMS antibody
    • Mesenchyme to epithelium transition protein with SH3 domains 1 antibody
    • METS 1 antibody
    • Mets1 antibody
    see all

Anti-CD2AP antibody images

  • ab84829 at a 1/200 dilution showing cytoplasmic staining of CD2AP in formalin-fixed, paraffin-embedded kidney tissue sections by immunohistochemistry.
  • ICC/IF image of ab84829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84829, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD2AP antibody (ab84829)

This product has been referenced in:
  • Huang G  et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . Read more (PubMed: 28112381) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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