Overview

  • Product name
  • Description
    Goat polyclonal to CD2BP2
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    PKRKVTFQGVGDE

    , corresponding to N terminal amino acids 2-14 of Human CD2BP2.

  • Positive control
    • W: Human lymph node, thymus and tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide.
    Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

Target

Images

  • ab32899 (5µg/ml) shows staining of CD2BP2 in paraffin embedded Human Spleen following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows nucleolar staining in select splenocytes.

  • Anti-CD2BP2 antibody (ab32899) at 0.3 µg/ml + 35ug of human thymus lysate in RIPA buffer

    Predicted band size: 38 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.
    Detected by chemiluminescence.

References

ab32899 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab32899.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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