Anti-CD3 + CD4 antibody [BB12 + EDU-2] (Dual color: FITC + PE) (ab1199)

Overview

  • Product nameAnti-CD3 + CD4 antibody [BB12 + EDU-2] (Dual color: FITC + PE)
    See all CD3 + CD4 primary antibodies
  • Description
    Mouse monoclonal [BB12 + EDU-2] to CD3 + CD4 (Dual color: FITC + PE)
  • ConjugationDual color: FITC + PE
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notesThis product is a mixture of two clones: CD3 = Clone:BB12, Isotype:IgG1, Label:FITC CD4 = Clone:EDU-2, Isotype:IgG2a, Label:PE

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • Clonality Monoclonal
  • Clone numberBB12 + EDU-2
  • Myelomaunknown
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesCounting and identification of T lymphocytes (amount CD3+CD4+ and CD3+CD4- populations) in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    Counting of proportion of T helper/inducer subtype as to total T lymphocytes (ratio CD3+CD4+ / CD3+ total lymphocytes).
    Characterization of subtypes of T cell leukaemias and lymphomas.
    Studies of HIV (Human Immunodeficiency Virus) infection.

    CD3(FITC) / CD4 (PE) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl of CD3(FITC) / CD4 (PE) is sufficient for labelling 1x106 cells.
  • Target

    • RelevanceCD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
    • Cellular localizationMembrane: single pass type I membrane protein.
    • Database links
    • Alternative names
      • CD3-GAMMA antibody
      • CD3D antibody
      • CD3E antibody
      • CD3G antibody
      • CD4 antibody
      • Leu3 antibody
      • T-cell antigen receptor complex antibody
      • T3G antibody
      see all

    References for Anti-CD3 + CD4 antibody [BB12 + EDU-2] (Dual color: FITC + PE) (ab1199)

    This product has been referenced in:
    • Cabrera-Socorro A  et al. Multiple isoforms of the tumor protein p73 are expressed in the adult human telencephalon and choroid plexus and present in the cerebrospinal fluid. Eur J Neurosci 23:2109-18 (2006). Read more (PubMed: 16630058) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"