General notesThis Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE/Cy7-conjugated antibodies, always protect samples from light.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: Less than or equal to 0.09% Sodium Azide
Constituents: PBS, pH 7.2 with BSA and/or Gelatin for protein stabilization
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5-1µg for 105-8 cells. Staining the cell sample in a final volume of 100µl is recommended.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Flow cytometry staining of C57BL/6 splenocytes with anti-CD45R (B220) PE and 0.5 µg of Armenian Hamster IgG Isotype Control PE/Cy7 (left) or 0.5 µg of ab95506 (right). Cells in the lymphocyte gate were used for analysis.
References for Anti-CD3 antibody [145-2C11] (PE/Cy7 ®) (ab95506)
This product has been referenced in:
Geng H et al. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer118:2657-64 (2006).
Read more (PubMed: 16425224) »
Krieg C et al. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol175:6420-7 (2005).
Read more (PubMed: 16272294) »