The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. PubMed: 18628996
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 23 kDa.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The recommended starting incubation time is 10min.
Use at an assay dependent concentration. PubMed: 20126467
Use at an assay dependent concentration. PubMed: 23874197
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody (ab5690)This image is courtesy of an Abreview submitted by Dr Alexandre Garin
ab5690 at 1/100 staining mouse lymph node tissue sections by IHC-Fr. The tissue was acetone fixed and blocked with serum before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
No positive immunostaining for CD3ε, a pan T cell marker (ab5690), was detected in the corneas of scrambled siRNA-treated mice (A) at 5 days p.i. In contrast, positive immunostaining (red) was observed in the peripheral cornea of HIF-1α silenced animals (B). The control sections shown in (C) and (D) were immunostained with species-specific IgG and were positive for SYTOX Green nuclear stain only. Images shown are representative of three independent experiments each with three mice per group. Magnification = 180×; inset = 335×.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)This image is courtesy of an Abreview submitted by Dr Reza Jalili
ab5690 staining rat spleen tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in sodium citrate buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0) prior to blocking in 5% normal goat serum + 1% BSA for 2 hours at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. A biotinylated goat anti-rabbit antibody, diluted 1/100, was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)Image courtesy of an anonymous Abreview.
ab5690 staining CD3 in the lymphatic nodule of rat spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 1% BSA for 25 minutes at 20°C followed by incubation with the primary antibody at a 1/100 dilution for 1 hour at 20°C. A biotin-conjugated goat anti-rat polyclonal was used undiluted as the secondary antibody. CD3 positive cells are mainly distributed in PALS (T-cell region), while sporadic CD3 positive cells are identified in lymphoid follicle and germinal center (B-cell region).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)This image is courtesy of an Anonymous Abreview.
ab5690 staining CD3 in human lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer pH 6.0, prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.