The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes1. Counting and identification of mature T lymphocytes in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
2. In combination with other monoclonal antibodies, this antibody may also be useful for thediagnosis of T cell leukaemias and lymphomas.
CD3 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD3 is sufficient for labelling of 1x106 cells.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.