FITC Anti-CD3 epsilon antibody [C363.29B] (ab24948)
Key features and details
- FITC Rat monoclonal [C363.29B] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2c
Overview
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Product name
FITC Anti-CD3 epsilon antibody [C363.29B]
See all CD3 epsilon primary antibodies -
Description
FITC Rat monoclonal [C363.29B] to CD3 epsilon -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.
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Epitope
This antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex. -
Positive control
- Flow Cyt: Cells from BALB/c mouse mesenteric lymph nodes. Mouse splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
C363.29B -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 3µg for 106 cells.
In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided). |
Notes |
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Flow Cyt
Use 3µg for 106 cells. In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided). |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.
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Ab24948 (3µg for 106 cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab24948 has been referenced in 3 publications.
- Mohamad NE et al. Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo. Nutr Metab (Lond) 16:49 (2019). PubMed: 31372176
- Zulkawi N et al. In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™. BMC Complement Altern Med 17:344 (2017). Flow Cyt ; Mouse . PubMed: 28666436
- Muraille E et al. Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major. PLoS Pathog 6:e1001154 (2010). PubMed: 20976202