The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FACS: 1/5 - 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul. Membrane permeabilisation is required for this application.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The CD3 complex mediates signal transduction.
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.