The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We suggest an incubation period of 30-60 minutes at room temperature.
Use at an assay dependent concentration.
The CD3 complex mediates signal transduction.
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
References for Anti-CD3 antibody [F7.2.38] (ab17143)
This product has been referenced in:
Cox AR et al. Resolving Discrepant Findings on ANGPTL8 in ß-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy. PLoS One11:e0159276 (2016).
Read more (PubMed: 27410263) »
Keijsers RR et al. In Vivo Induction of Cutaneous Inflammation Results in the Accumulation of Extracellular Trap-Forming Neutrophils Expressing ROR?t and IL-17. J Invest DermatolN/A:N/A (2013).
Read more (PubMed: 24317395) »