Anti-CD3 antibody [KT3] (Phycoerythrin) (ab22268)

Overview

  • Product nameAnti-CD3 antibody [KT3] (Phycoerythrin)
    See all CD3 primary antibodies
  • Description
    Rat monoclonal [KT3] to CD3 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody recognises the mouse CD3 antigen which is expressed by mature T cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Dog
  • Immunogen

    Full length native protein (purified) corresponding to Mouse CD3.

  • Positive control
    • FC: Mouse spleen, Canine blood leucocytes, Human PBMC
  • General notes

    Purified IgG conjugated to R. Phycoerythrin (RPE).

Properties

Applications

Our Abpromise guarantee covers the use of ab22268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similaritiesContains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Anti-CD3 antibody [KT3] (Phycoerythrin) images

  • ab22268 staining CD3 in mouse spleen cells by Flow Cytometry analysis.
  • ab22268 used in Flow Cytometry.
    Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab22268 used at a 1/100 dilution for 1 hour at 4°C.

    The gating strategy used was for lymphocytes.

    See Abreview

  • Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
    Gating Strategy: Lymphocytes

References for Anti-CD3 antibody [KT3] (Phycoerythrin) (ab22268)

ab22268 has not yet been referenced specifically in any publications.

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Unfortunately the lab didn't have much information to add regarding this your experimentation. Because the absorbance wavelengths of PE and PerCP are right on top of one another, the only action we know of that ca...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rabbit Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
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Abcam user community

Verified customer

Submitted Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 25 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse spleen)
Specification mouse spleen
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 17 2012

Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Permeabilization No
Gating Strategy lymphocytes
Username

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Verified customer

Submitted Nov 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from lymph nodes of immunized mouse)
Specification cells from lymph nodes of immunized mouse
Preparation Cell harvesting/tissue preparation method: mechanical disruption
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Permeabilization No
Gating Strategy on alive cells
Username

Abcam user community

Verified customer

Submitted Sep 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Aug 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"