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Full length native protein (purified) corresponding to Mouse CD3.
Purified IgG conjugated to R. Phycoerythrin (RPE).
Our Abpromise guarantee covers the use of ab22268 in the following tested applications.
|Flow Cyt||Use 10µl for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
Gating Strategy: Lymphocytes
ab22268 has not yet been referenced specifically in any publications.