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Full length native protein (purified) corresponding to Mouse CD3.
Purified IgG conjugated to R. Phycoerythrin (RPE).
Our Abpromise guarantee covers the use of ab22268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab22268 at a dilution of (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. Cell separation was achieved by FICOLL medium with PBS + 0.5% BSA.
Gating Strategy: Lymphocytes
ab22268 has not yet been referenced specifically in any publications.