SpecificityThis antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Activates T cells in vitro; has a mitogenic effect on peripheral T lymphocytes.
Use at an assay dependent concentration.
Use 0.01µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
References for Anti-CD3 antibody [MEM-57] (ab8090)
This product has been referenced in:
Lipp AM et al. Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells. PLoS One9:e85934 (2014).
Read more (PubMed: 24454946) »
Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog10:e1004189 (2014).
Read more (PubMed: 24991932) »