Overview

  • Product name
    Anti-CD3 antibody [PS1]
    See all CD3 primary antibodies
  • Description
    Mouse monoclonal [PS1] to CD3
  • Specificity
    This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prokaryotic fusion protein corresponding to the epsilon chain of CD3

  • Positive control
    • Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
  • General notes
    This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

Properties

Applications

Our Abpromise guarantee covers the use of ab699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 21654633
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Involvement in disease
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Anti-CD3 antibody [PS1] images

  • ab699 staining CD3 in paraffin-embedded human colorectal mucosa tissue sections by IHC-P. Tissue was fixed with formaldehyde and blocked with 10% Goat serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/50 in PBS/BSA/Tween) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti mouse/rabbit polymer was used as secondary antibody.

    See Abreview

  • ab699 staining CD3 in human colon tissue by immunohistochemistry. Tissue was fixed in acetone, blocked using 10% donkey serum/PBS for 30 minutes at 21°C and then incubated with ab699 at a 1/40 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor® 555 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution. CD3 in red nuclear counterstaining with DAPI in blue.

    See Abreview

  • All lanes : Anti-CD3 antibody [PS1] (ab699) at 1/1000 dilution

    Lane 1 : Positive control - CD3 protein
    Lane 2 : Whole cell Jurkat lysate
    Lane 3 : Whole cell PBMCs lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat polyclonal anti-mouse CD3 HRP at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 minutes

    Gel running conditions: Reduced non-denaturing (native) (5-16% gels).

    Incubation time: 16 hours at 4°C in 5% BSA/ TBST.

     

    See Abreview

  • Methanol fixed human lymphocyte cells stained for CD3 (red) using ab699 at a 1/75 dilution in ICC/IF. Blocked for 30 minutes at RT using 4% BSA. Incubation time: 16 hours at 4°C in PBS/4% BSA. Secondary antibody: Goat polyclonal anti-mouse TexasRed® at a 1/100 dilution.

    See Abreview

  • ab669 staining CD3 in human skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in methanol, blocked using 4% BSA for 30 minutes at room temperature and then incubated with ab699 at a 1/75 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-mouse polyclonal used at 10µg/ml.

    See Abreview

References for Anti-CD3 antibody [PS1] (ab699)

This product has been referenced in:
  • Milovanovic J  et al. Murine Cytomegalovirus Infection Induces Susceptibility to EAE in Resistant BALB/c Mice. Front Immunol 8:192 (2017). IHC-P ; Mouse . Read more (PubMed: 28289417) »
  • Shi H  et al. Long non-coding RNA expression profile in minor salivary gland of primary Sjögren's syndrome. Arthritis Res Ther 18:109 (2016). IHC ; Human . Read more (PubMed: 27188286) »

See all 6 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Human Sarcoma
Blocking step
innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Sample
Human Cell lysate - whole cell (Jurkats and PBMCS)
Specification
Jurkats and PBMCS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2013

Here is a link to our protocol for sodium azide removal

http://www.abcam.com/index.html?pageconfig=resource&rid=13869

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lymphocytes)
Specification
Lymphocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Specification
Human peripheral blood mononuclear cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 25 2013

Vielen Dank für Ihre Anfrage.

Die Konzentration für den ab699 Anti-CD3 antibody [PS1] mit dem Lot GR95849-1, welches Sie erhalten haben, beträgt 80ug/ml.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nic...

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spleen whole extract)
Loading amount
100 µg
Specification
Spleen whole extract
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human peripheral blood mononuclear cells)
Loading amount
25 µg
Specification
Human peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2012

1-10 of 24 Abreviews or Q&A

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