Non-Abcam antibody was used: anti Rabbit IgG Host species: Goat Clonality: Polyclonal Conjugation: Biotin
Cryostat sections ( 10 microns thick) of fresh frozen spleen were dried overnight. They were fixed in 10% Formalin in PBS pH7 for 15 minutes.
After secondary antibody incubation ( Gt anti Rb -biotin) , stAvidin-Alexa 594 was applied.
Mr. Carl Hobbs
Submitted Dec 21 2016
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