Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [SP7]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/100

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Primary antibody dilution factor, using my standard stABCpx- DAB method, is not as high as that obtained when visualising CD3 on cryostat section, using indirect immunofluorescence. Thus my "Good" rating. However, specificity is excellent. NB: Use Abcam's DAB reagent (ab103723) to increase the dilution factor of primary antibody x2/3, imho.
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 21 2016

Sign up