Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [SP7]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Epididymal Fat Pad)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton X-100
Specification
Mouse Epididymal Fat Pad
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST with BSA and goat serum
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor­ 568

Additional data

PubMed ID
28486786: View
Username

Ms. Ying Li

Verified customer

Submitted Nov 23 2017

Sign up