Overview

  • Product nameAnti-CD3 antibody [SP7], prediluted
    See all CD3 primary antibodies
  • Description
    Rabbit monoclonal [SP7] to CD3, prediluted
  • Specificityab21703 recognises CD3 epsilon chain. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Cynomolgus Monkey
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Macaque Monkey, Woodchuck
  • Immunogen

    Synthetic peptide:

    KAKAKPVTRGAGA

    , corresponding to amino acids 156-168 of Human CD3, epsilon chain.

  • Positive control
    • Tonsil tissue.
  • General notes


    This antibody is suitable for staining normal and neoplastic T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab21703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25. Predicted molecular weight: 19 kDa.
IHC-Fr 1/1.

Acetone fixed.

IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similaritiesContains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Anti-CD3 antibody [SP7], prediluted images

  • Anti-CD3 antibody [SP7], prediluted (ab21703) at 1/25 dilution + Jurkat cell lysate

    Predicted band size : 19 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD3 with ab21703.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining CD3 with ab21703.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue, staining CD3 with ab21703.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, staining CD3 with ab21703.

References for Anti-CD3 antibody [SP7], prediluted (ab21703)

This product has been referenced in:
  • Sakai K  et al. Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. J Virol 87:1105-14 (2013). IHC-P ; Cynomolgus Monkey . Read more (PubMed: 23135729) »
  • Turner A  et al. Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty. Eur Urol 59:447-54 (2011). Pig . Read more (PubMed: 21195539) »

See all 2 Publications for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Thank you for contacting us. As discussed on the phone this antibody is supplied as tissue culture supernatant, the exact concentration of the solution is therefore not know. The product is however prediluted and can therefore be used neat with your IH...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"