Anti-CD3 antibody [SPV-T3b] (ab34182)

Overview

  • Product nameAnti-CD3 antibody [SPV-T3b]
    See all CD3 primary antibodies
  • Description
    Mouse monoclonal [SPV-T3b] to CD3
  • SpecificityThis antibody is specific for CD3 at a MW of 19-29 kDa.
  • Tested applicationsSuitable for: Flow Cyt, Blocking, IHC-Fr, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HG-38.

Properties

Applications

Our Abpromise guarantee covers the use of ab34182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.
IHC-Fr 1/10 - 1/20.
ICC/IF 1/10 - 1/20.
WB 1/1000. Predicted molecular weight: 19-29 kDa.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    • Sequence similaritiesContains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • Cd247 antibody
      • CD247 antigen antibody
      • CD247 antigen, zeta subunit antibody
      • CD247 molecule antibody
      • CD3 antibody
      • CD3 antigen, delta subunit antibody
      • CD3 delta antibody
      • CD3 epsilon antibody
      • CD3 eta antibody
      • CD3 gamma antibody
      • CD3 molecule delta polypeptide antibody
      • CD3 molecule, epsilon polypeptide antibody
      • CD3 molecule, gamma polypeptide antibody
      • CD3 zeta antibody
      • CD3-DELTA antibody
      • CD3d antibody
      • CD3D antigen delta polypeptide antibody
      • CD3d antigen, delta polypeptide (TiT3 complex) antibody
      • CD3d molecule delta antibody
      • CD3d molecule delta CD3 TCR complex antibody
      • CD3d molecule, delta (CD3-TCR complex) antibody
      • CD3D_HUMAN antibody
      • CD3E antibody
      • CD3e antigen antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule epsilon CD3 TCR complex antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3epsilon antibody
      • CD3G antibody
      • CD3g antigen antibody
      • CD3G antigen gamma polypeptide antibody
      • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
      • CD3g molecule gamma antibody
      • CD3g molecule gamma CD3 TCR complex antibody
      • CD3g molecule, gamma (CD3-TCR complex) antibody
      • CD3H antibody
      • CD3Q antibody
      • CD3Z antibody
      • CD3zeta antibody
      • Ctg3 antibody
      • FLJ17620 antibody
      • FLJ17664 antibody
      • FLJ18683 antibody
      • FLJ79544 antibody
      • FLJ94613 antibody
      • IMD19 antibody
      • Leu-4 antibody
      • MGC138597 antibody
      • OKT3, delta chain antibody
      • OTTHUMP00000032544 antibody
      • T cell receptor antibody
      • T cell receptor T3 delta chain antibody
      • T cell receptor T3 gamma chain antibody
      • T cell receptor T3 zeta chain antibody
      • T cell receptor zeta chain antibody
      • T cell surface glycoprotein CD3 antibody
      • T cell surface glycoprotein CD3 delta chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T cell surface glycoprotein CD3 gamma chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, delta subunit of T3 antibody
      • T-cell antigen receptor complex, epsilon subunit of T3 antibody
      • T-cell antigen receptor complex, gamma subunit of T3 antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 delta chain antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 delta chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3 antibody
      • T3d antibody
      • T3e antibody
      • T3g antibody
      • T3z antibody
      • TCRE antibody
      • TCRk antibody
      • Tcrz antibody
      • TCRzeta antibody
      see all

    Anti-CD3 antibody [SPV-T3b] images

    • Human peripheral blood lymphocytes stained with ab34182 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab34182, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    • ab34182 staining CD3 in human stomach tissue by Immunohistochemistry.
      Tissue was fixed in acetone, blocked using 10% serum for 30 minutes at 21°C and then incubated with ab34182 at a 1/20 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution.
      ab34182 staining CD3 in human stomach tissue by Immunohistochemistry.
      Tissue was fixed in acetone, blocked using 10% serum for 30 minutes at 21°C and then incubated with ab34182 at a 1/20 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 555 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution.

      See Abreview

    • Anti-CD3 antibody [SPV-T3b] (ab34182) at 1 µg/ml + whole tissue lysate prepared from human colon at 50 µg

      Secondary
      HRP conjugated goat anti-mouse polyclonal at 1/3000 dilution
      Developed using the ECL technique

      Predicted band size : 19-29 kDa
      Observed band size : 29 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 seconds

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-CD3 antibody [SPV-T3b] (ab34182)

    ab34182 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (skin)
    Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
    Loading amount 15 µg
    Specification skin
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Submitted Apr 14 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample Human Cell (PBMCs)
    Specification PBMCs
    Permeabilization No
    Fixative Methanol
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    Submitted Mar 23 2015

    Application Flow Cytometry
    Fixation no fixation
    Permeabilization No
    Sample Human Cell (PBMCs)
    Specification PBMCs
    Gating Strategy lympho and mono gate
    Preparation Cell harvesting/tissue preparation method: Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation
    Sample buffer: PBS with human IgG (Fc-block)
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    Submitted Mar 23 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample Human Tissue sections (skin)
    Specification skin
    Permeabilization No
    Fixative Acetone
    Username

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    Verified customer

    Submitted Mar 23 2015

    Here is a link to our protocol for sodium azide removal

    http://www.abcam.com/index.html?pageconfig=resource&rid=13869

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (human colon lysate)
    Loading amount 50 µg
    Specification human colon lysate
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (human stomach section)
    Specification human stomach section
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

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    Verified customer

    Submitted Dec 14 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"