Specificityab106224 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. It reacts with the epsilon chain of the CD3 complex.
HLDA I; WS Code T 3
HLDA III; WS Code T 126
HLDA III; WS Code T 471
HLDA VI; WS Code T 6T-CD3.1
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Flow cytometry of human peripheral blood lymphocytes labeling CD3 with ab106224 at 0.25µg (solid line), or Mouse IgG1 isotype control at 0.25µg (dashed line).
References for Anti-CD3 antibody [UCHT1] (Allophycocyanin) (ab106224)
This product has been referenced in:
Siegers GM et al. Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med204:2537-44 (2007).
Read more (PubMed: 17923503) »
Demedts IK et al. Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol32:177-84 (2005).
Read more (PubMed: 15576669) »