The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Flow cytometric staining of normal Human peripheral blood cells with PE-Cy5 Mouse IgG1, K Isotype Control (open histogram) or ab95510 (colored histogram). Cells in the lymphocyte gate were used for analysis.
References for Anti-CD3 antibody [UCHT1] (PE/Cy5®) (ab95510)