Anti-CD3 + CD19 antibody [BB12 + CB19] (Dual color: FITC + PE) (ab1225)

Overview

  • Product nameAnti-CD3 + CD19 antibody [BB12 + CB19] (Dual color: FITC + PE)
  • Description
    Mouse monoclonal [BB12 + CB19] to CD3 + CD19 (Dual color: FITC + PE)
  • ConjugationDual color: FITC + PE
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notesThis product is a mixture of two clones: CD3 = Clone:BB12, Isotype:IgG1, Label:FITC CD19 = Clone:CB19, Isotype:IgG1, Label:PE


    CD3: Human T-cell associated antigen (Mw = 22-28kDa) is part of a structure containing at least five subunits which are non-covalently associated with either TCR alpha/beta or TCR gamma/delta. It is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. This antigen is never expressed on B or NK cells. CD19: This antigen (MW = 95kDa) is present on 10-15% of human peripheral blood lymphocytes and approximately 60% of splenic lymphocytes. It is not expressed on T lymphocytes, granulocytes, activated T cells or monocytes. Moreover, CD19 is present on principal acute and chronic B cell leukemias and lymphomas.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • Primary antibody notesCD3: Human T-cell associated antigen (Mw = 22-28kDa) is part of a structure containing at least five subunits which are non-covalently associated with either TCR alpha/beta or TCR gamma/delta. It is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. This antigen is never expressed on B or NK cells. CD19: This antigen (MW = 95kDa) is present on 10-15% of human peripheral blood lymphocytes and approximately 60% of splenic lymphocytes. It is not expressed on T lymphocytes, granulocytes, activated T cells or monocytes. Moreover, CD19 is present on principal acute and chronic B cell leukemias and lymphomas.
  • ClonalityMonoclonal
  • Clone numberBB12 + CB19
  • Myelomaunknown
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Target

  • RelevanceT cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
  • Cellular localizationMembrane; single-pass type I membrane protein.
  • Database links
  • Alternative names
    • CD19 antigen antibody
    • CD19 molecule antibody
    • CD3 DELTA antibody
    • CD3 EPSILON antibody
    • CD3 GAMMA antibody
    • CD3D antibody
    • CD3D molecule antibody
    • CD3E antibody
    • CD3E molecule antibody
    • CD3G antibody
    • CD3G molecule antibody
    see all

References for Anti-CD3 + CD19 antibody [BB12 + CB19] (Dual color: FITC + PE) (ab1225)

ab1225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"