Anti-CD3 epsilon antibody [145-2C11] (ab25468)

Overview

  • Product nameAnti-CD3 epsilon antibody [145-2C11]
    See all CD3 epsilon primary antibodies
  • Description
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon
  • Specificityab25468 recognises CD3 epsilon.
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, Celluar Activation, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: H2Kb specific mouse cytotoxic T lymphocyte clone BM10 37.

Properties

Applications

Our Abpromise guarantee covers the use of ab25468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
IP
Celluar Activation
Functional Studies
  • Application notesCellular activation: Use at an assay dependent dilution.
    Note: In the presence of Fc receptor bearing accessory cells, soluble ab25468 can activate primed and naive T cell in vitro. Immobilized ab25468 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25468 may result in apoptotic cell death.
    Flow Cyt: Use at an assay dependent dilution.
    FuncS: Use at an assay dependent dilution.
    Note: ab25468 can be used for in vitro depletion of CD3+ cells, for induction of redirected lysis of specific target cells by CTL clones and for blocking lysis of specific target cells by antigen specific CTL's.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    References for Anti-CD3 epsilon antibody [145-2C11] (ab25468)

    ab25468 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    The following paper may be helpful in determining the binding affinity of this antibody. Basically, this information can be measured in an indirectELISA assay using the antibody and a purified antigen such as ab11...

    Read More

    Thank you for your enquiry. Unfortunately, we are unable to sub-isotype Hamster IgG. We are only able to provide the isotype as being Hamster IgG.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"