Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) (ab25172)

Overview

  • Product nameAnti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide)
    See all CD3 epsilon primary antibodies
  • Description
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (Biotin-maleimide)
  • ConjugationBiotin-maleimide
  • Specificityab25172 recognises Mouse CD3 epsilon (Mr 25 kDa).
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, IP, WB, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37.

  • Epitopeab25172 recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • General notes


    In the presence of Fc receptor-bearing accessory cells, soluble ab25172 can activate primed and naïve T cell in vitro and can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25172 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesIn the presence of Fc receptor-bearing accessory cells, soluble ab25172 can activate primed and naïve T cell in vitro and can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25172 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
  • ClonalityMonoclonal
  • Clone number145-2C11
  • IsotypeIgG
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

In vitro depletion of CD3+ cells.

Flow Cyt Use 3µg for 106 cells.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
Cellular Activation Use at an assay dependent concentration.

In vitro activation of T cells.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

References for Anti-CD3 epsilon antibody [145-2C11] (Biotin-maleimide) (ab25172)

This product has been referenced in:
  • Mira E  et al. Statins induce regulatory T cell recruitment via a CCL1 dependent pathway. J Immunol 181:3524-34 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18714025) »
  • Leo O  et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A 84:1374-8 (1987). Read more (PubMed: 2950524) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab25172.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"