Anti-CD3 epsilon antibody [145-2C11] (FITC) (ab24947)

Overview

  • Product nameAnti-CD3 epsilon antibody [145-2C11] (FITC)
    See all CD3 epsilon primary antibodies
  • Description
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab24947 recognises CD3 epsilon chain.
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

  • Positive control
    • BALB/c mouse spleen cells.
  • General notes


    ab24947 can induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen specific CTL’s. Immobilized ab24947 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab24947 may result in apoptotic cell death.

Properties

Applications

Our Abpromise guarantee covers the use of ab24947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 3µg for 106 cells. ab18473-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
Functional Studies Use at an assay dependent concentration.

In vitro depletion of CD3 positive cells, and for in vitro activation of T cells.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [145-2C11] (FITC) images

  • BALB/c spleen cells were double-stained with hamster anti mouse CD3 epsilon FITC conjugated (ab24947) and rat anti mouse CD19 RPE. Lymphocytes were gated and analyzed on a flow cytometer.
  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (FITC) ab24947 (1 µg/106 cells), and Rat Anti-Mouse CD19-PE.

  • ab24947 staining mouse BALB/c spleen cells by flow cytometry. 1µg antibody was used for 106 cells.

References for Anti-CD3 epsilon antibody [145-2C11] (FITC) (ab24947)

ab24947 has not yet been referenced specifically in any publications.

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