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Tissue/ cell preparation: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
ab24947 can induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen specific CTL’s. Immobilized ab24947 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab24947 may result in apoptotic cell death.
Our Abpromise guarantee covers the use of ab24947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 3µg for 106 cells. ab18473-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
|Functional Studies||Use at an assay dependent concentration.
In vitro depletion of CD3 positive cells, and for in vitro activation of T cells.
Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (FITC) ab24947 (1 µg/106 cells), and Rat Anti-Mouse CD19-PE.
ab24947 staining mouse BALB/c spleen cells by flow cytometry. 1µg antibody was used for 106 cells.
ab24947 has not yet been referenced specifically in any publications.