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H-2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.
In the presence of Fc receptor-bearing accessory cells, soluble ab25453 can activate primed and naïve T cell in vitro. ab25453 can also induce redirected lysis ofspecific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25453 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25453 may result in apoptotic cell death.
Our Abpromise guarantee covers the use of ab25453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
In vitro depletion of CD3+ cells.
|Flow Cyt||Use 1µg for 106 cells. Ab157805-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
ab157805-Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
|IP||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration.
ab25453 has not yet been referenced specifically in any publications.