Anti-CD3 epsilon antibody [145-2C11] (PE/Cy5®) (ab25453)

Overview

  • Product nameAnti-CD3 epsilon antibody [145-2C11] (PE/Cy5®)
    See all CD3 epsilon primary antibodies
  • Description
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, WB, IP, Celluar Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    H-2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.

  • EpitopeThe 145-2C11 monoclonal antibody recognizes an epitope on the 25kD e chain of the CD3/TCR complex.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In the presence of Fc receptor-bearing accessory cells, soluble ab25453 can activate primed and naïve T cell in vitro. ab25453 can also induce redirected lysis ofspecific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25453 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25453 may result in apoptotic cell death.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, 0.2% Gelatin, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesIn the presence of Fc receptor-bearing accessory cells, soluble ab25453 can activate primed and naïve T cell in vitro. ab25453 can also induce redirected lysis ofspecific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized ab25453 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25453 may result in apoptotic cell death.
  • ClonalityMonoclonal
  • Clone number145-2C11
  • IsotypeIgG
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

In vitro depletion of CD3+ cells.

Flow Cyt Use 1µg for 106 cells. Ab157805-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

ab157805-Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent concentration.
Celluar Activation Use at an assay dependent concentration.

T cells.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

References for Anti-CD3 epsilon antibody [145-2C11] (PE/Cy5®) (ab25453)

ab25453 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"