Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) (ab25665)

Overview

  • Product nameAnti-CD3 epsilon antibody [145-2C11] (Phycoerythrin)
    See all CD3 epsilon primary antibodies
  • Description
    Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody recognises CD3 epsilon.
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: H2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.

  • EpitopeThis antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • General notes


    In the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesIn the presence of Fc receptor bearing accessory cells, this antibody, in the soluble form, can activate primed and naïve T cell in vitro. This antibody also induces redirected lysis of specific target cells by CTL clones, and can block lysis of specific target cells by antigen specific CTL's. Immobilized ab25665 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by ab25665 may result in apoptotic cell death.
  • ClonalityMonoclonal
  • Clone number145-2C11
  • IsotypeIgG
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
IP
Functional Studies
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution. Ab25665 can be used for in vitro depletion of CD3 positive cells and for in vitro activation of T cells.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3E antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) images

    • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) ab25665 (1 µg/106 cells), and Rat Anti-Mouse CD19-FITC.

    References for Anti-CD3 epsilon antibody [145-2C11] (Phycoerythrin) (ab25665)

    ab25665 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25665.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"