Overview

  • Product nameAnti-CD3 epsilon antibody [500-A2] (Biotin)
    See all CD3 epsilon primary antibodies
  • Description
    Syrian Hamster monoclonal [500-A2] to CD3 epsilon (Biotin)
  • ConjugationBiotin
  • Specificityab25265 recognises CD3e.
  • Tested applicationsSuitable for: Flow Cyt, IP, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka).

  • Epitopeab25265 recognizes an epitope on the 25kD e chain of the CD3/TCR complex of mouse strains.

Properties

Applications

Our Abpromise guarantee covers the use of ab25265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Cellular Activation
  • Application notesFlow Cyt: Use 3µg for 106 cells.
    IP: Use at an assay dependent dilution.
    FACS (In vitro activation of T cells): Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [500-A2] (Biotin) images

    • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [500-A2] (Biotin) ab25265 (0.3 µg/106 cells) and Rat Anti-Mouse CD19-PE followed by Streptavidin-FITC.

    References for Anti-CD3 epsilon antibody [500-A2] (Biotin) (ab25265)

    This product has been referenced in:
    • Portoles P  et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol 142:4169-75 (1989). Read more (PubMed: 2470817) »
    • Havran WL  et al. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature 330:170-3 (1987). Read more (PubMed: 2959867) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"