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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.1% Sodium Azide
Concentration information loading...
Purity IgG fraction
Clone number 500-A2
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Flow Cyt: Use 3µg for 10 6 cells.
IP: Use at an assay dependent dilution.
FACS (In vitro activation of T cells): Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function The CD3 complex mediates signal transduction.
Sequence similarities Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.
Cellular localization Membrane.
Information by UniProt
CD3 epsilon antibody
CD3e antigen epsilon polypeptide (TiT3 complex) antibody
Anti-CD3 epsilon antibody [500-A2] (Biotin) images
Flow Cytometry - Anti-CD3 epsilon antibody [500-A2] (Biotin) (ab25265)
Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [500-A2] (Biotin) ab25265 (0.3 µg/10
6 cells) and Rat Anti-Mouse CD19-PE followed by Streptavidin-FITC.
References for Anti-CD3 epsilon antibody [500-A2] (Biotin) (ab25265)
This product has been referenced in:
et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol 142:4169-75 (1989).
Read more (PubMed: 2470817) »
et al. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature 330:170-3 (1987).
Read more (PubMed: 2959867) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"