Anti-CD3 epsilon antibody [500-A2] (Cy5 ®) (ab25586)

Overview

  • Product nameAnti-CD3 epsilon antibody [500-A2] (Cy5 ®)
    See all CD3 epsilon primary antibodies
  • Description
    Syrian Hamster monoclonal [500-A2] to CD3 epsilon (Cy5 ®)
  • ConjugationCy5 ®. Ex: 650nm, Em: 667nm
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka).

  • EpitopeThis antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of the mouse strains tested.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    ab25586 can be used In vitro to inhibit CTL-mediated lysis.

Properties

Applications

Our Abpromise guarantee covers the use of ab25586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Cellular Activation Use at an assay dependent concentration.

In vitro T Cell activation.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [500-A2] (Cy5 ®) images

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [500-A2] (Cy5) ab25586 (0.1 µg/106 cells) and Rat Anti-Mouse CD19-FITC.

References for Anti-CD3 epsilon antibody [500-A2] (Cy5 ®) (ab25586)

ab25586 has not yet been referenced specifically in any publications.

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