Anti-CD3 epsilon antibody [500-A2] (PE/Cy5®) (ab25530)

Overview

  • Product nameAnti-CD3 epsilon antibody [500-A2] (PE/Cy5®)
    See all CD3 epsilon primary antibodies
  • Description
    Syrian Hamster monoclonal [500-A2] to CD3 epsilon (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Specificityab25530 recognises Mouse CD3e, Mr 25 kDa
  • Tested applicationsSuitable for: Flow Cyt, IP, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka).

  • EpitopeThis antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab25530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Cellular Activation
  • Application notesCellular activation: Use at an assay dependent dilution.
    Flow Cyt: Use 1µg for 106 cells.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [500-A2] (PE/Cy5®) images

    • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [500-A2] (PE/Cy5) ab25530 (0.1 µg/106 cells) and Rat Anti-Mouse CD19-PE.

    References for Anti-CD3 epsilon antibody [500-A2] (PE/Cy5®) (ab25530)

    ab25530 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"