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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD3 epsilon antibody
See all CD3 epsilon products (44) ...
Description
Rabbit polyclonal to CD3 epsilon
Specificity
ab26249 reacts with intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Horse, Cat, Dog, Human, Monkey, Baboon
Immunogen
Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon
KAKAKPVTRG AGA
Positive control
Tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The CD3 complex mediates signal transduction.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP07766
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 3E antibody
- CD3 TCR complex antibody
- CD3E antibody
see all
Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: Q5R1Q1 Cat
- SwissProt: P27597 Dog
- SwissProt: P07766 Human
- Unigene: 3003 Human
Anti-CD3 epsilon antibody images:
Western blot - CD3 epsilon antibody (ab26249)
All lanes : Anti-CD3 epsilon antibody (ab26249) at 1/500 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD3 epsilon antibody(ab26249)
ab26249 (2µg/ml) staining CD3 epsilon in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-CD3 epsilon antibody (ab26249)
ab26249 has not yet been referenced specifically in any publications.
Publishing research using ab26249? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"