Anti-CD3 epsilon antibody (ab26249)




Our Abpromise guarantee covers the use of ab26249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The CD3 complex mediates signal transduction.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody images

    • All lanes : Anti-CD3 epsilon antibody (ab26249) at 1/500 dilution

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
    • ab26249 (2µg/ml) staining CD3 epsilon in human spleen.

      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References for Anti-CD3 epsilon antibody (ab26249)

    ab26249 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry. The concentration of the antibody is 100 ug/ml. I hope this information helps. Please do not hesitate to contact us again if you need anything further.


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