CD3 epsilon antibody (ab26249)

Product Name 

CD3 epsilon antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CD3 epsilon

Immunogen 

Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon
BLAST 'KAKAKPVTRGAGA' with or

Reacts with 
(species key)

Hu, Bb, Cat, Dog, Hrs, Mk

Specificity 

ab26249 reacts with intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD

Relevance 

CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

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