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Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.
Our Abpromise guarantee covers the use of ab24948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 3µg for 106 cells.
In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided).
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.