Anti-CD3 epsilon antibody [C363.29B] (FITC) (ab24948)

Overview

  • Product nameAnti-CD3 epsilon antibody [C363.29B] (FITC)
    See all CD3 epsilon primary antibodies
  • Description
    Rat monoclonal [C363.29B] to CD3 epsilon (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody recognises CD3 epsilon chain.
  • Tested applicationsSuitable for: Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.

  • EpitopeThis antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex.
  • Positive control
    • Cells from BALB/c mouse mesenteric lymph nodes for Immunofluorescence.

Properties

Applications

Our Abpromise guarantee covers the use of ab24948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 3µg for 106 cells.

In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided).
ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [C363.29B] (FITC) images

  • Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.

  • Ab24948 (3µg for 106 cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.

References for Anti-CD3 epsilon antibody [C363.29B] (FITC) (ab24948)

This product has been referenced in:
  • Muraille E  et al. Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major. PLoS Pathog 6:e1001154 (2010). Read more (PubMed: 20976202) »

See 1 Publication for this product

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