Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5®) (ab25531)

Overview

  • Product name
    Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5®)
    See all CD3 epsilon primary antibodies
  • Description
    Rat monoclonal [C363.29B] to CD3 epsilon (PE/Cy5®)
  • Conjugation
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitope
    This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, 0.20% Gelatin, PBS and stabilising agent.
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
  • Clonality
    Monoclonal
  • Clone number
    C363.29B
  • Isotype
    IgG2c
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent dilution.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5®) images

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5) ab25531 (1 µg/106cells) and Rat Anti-Mouse CD19-PE.

References for Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5®) (ab25531)

This product has been referenced in:
  • Carrithers MD  et al. Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment. Brain 123 ( Pt 6):1092-101 (2000). Read more (PubMed: 10825349) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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