Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (ab25469)

Overview

  • Product name
    Anti-CD3 epsilon antibody [C363.29B (YCD3-1)]
    See all CD3 epsilon primary antibodies
  • Description
    Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitope
    This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.
  • General notes


    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).
  • Clonality
    Monoclonal
  • Clone number
    C363.29B (YCD3-1)
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

References

ab25469 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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