Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (FITC) (ab25660)


  • Product name
    Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (FITC)
    See all CD3 epsilon primary antibodies
  • Description
    Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cells in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitope
    This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.
  • General notes

    The antibody is cytolytic, is easily used for cell surface staining and is a good immunoprecipitating antibody. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).



Our Abpromise guarantee covers the use of ab25660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 3µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.


  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (FITC) images

  • Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with RAnti-CD3 epsilon antibody [C363.29B (YCD3-1)] (FITC) ab25660 (1 µg/106cells) and Rat Anti-Mouse CD19-PE.
  • Cells from BALB/c mesenteric lymph nodes were double-stained with anti-Mouse CD3 epsilon-FITC (ab25660) at up to 3 ug/106 cells and anti-Mouse CD45R/B220-RPE, gated on small lymphocytes and analyzed using a flow cytometer.

References for Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (FITC) (ab25660)

ab25660 has not yet been referenced specifically in any publications.

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