Overview

  • Product nameAnti-CD3 epsilon antibody [E272]
    See all CD3 epsilon primary antibodies
  • Description
    Rabbit monoclonal [E272] to CD3 epsilon
  • Tested applicationsSuitable for: IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt or WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in C terminal cytoplasmic domain of human CD3 epsilon chain.

  • Positive control
    • IP: Jurkat cell lysate IHC-P: Human lymph node.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC 1/100 - 1/250.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or WB.
  • Target

    • FunctionThe CD3 complex mediates signal transduction.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Anti-CD3 epsilon antibody [E272] images

    • Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or PBS (lane 2: negative control), using ab32186 at a 1/50 dilution. The predicted band size of 23 kDa was observed.
    • Ab32186, at a 1/100 dilution, staining CD3 epsilon in paraffin embedded human lymph node tissue by Immunohistochemistry.

    References for Anti-CD3 epsilon antibody [E272] (ab32186)

    ab32186 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32186.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"