Recombinant
RabMAb

Anti-CD3 epsilon antibody [E292] (ab32327)

Overview

  • Product name
    Anti-CD3 epsilon antibody [E292]
    See all CD3 epsilon primary antibodies
  • Description
    Rabbit monoclonal [E292] to CD3 epsilon
  • Specificity
    ab32327 recognises CD3 espilon. The antibody does not cross-react with other subunits of CD3.
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in cytoplasmic domain of human CD3 epsilon

  • Epitope
    ab32327 reacts with an epitope located in the cytoplasmic region of CD3 epsilon.
  • Positive control
    • Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC 1/50.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function
      The CD3 complex mediates signal transduction.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Anti-CD3 epsilon antibody [E292] (ab32327) at 1/5000 dilution + Jurkat cell lysate

      Predicted band size : 23 kDa
      Observed band size : 23 kDa

    References

    ab32327 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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