Overview

  • Product nameAnti-CD3 epsilon antibody [PPT3] (FITC)
    See all CD3 epsilon primary antibodies
  • Description
    Mouse monoclonal [PPT3] to CD3 epsilon (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Celluar Activation, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Pig peripheral blood mononuclear cells.
  • General notes


    Porcine CD3e is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. ab24987 PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Properties

Applications

Our Abpromise guarantee covers the use of ab24987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Celluar Activation Use at an assay dependent concentration. In vitro activation of T cells.
Flow Cyt Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionThe CD3 complex mediates signal transduction.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Anti-CD3 epsilon antibody [PPT3] (FITC) images

  • Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll Hypaque density gradients and stained with ab24987 (1 µg/106 cells). Lymphocytes were gated and analyzed on a flow cytometer.

References for Anti-CD3 epsilon antibody [PPT3] (FITC) (ab24987)

This product has been referenced in:
  • Yang H  et al. Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes. Immunology 88:577-85 (1996). Read more (PubMed: 8881760) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"