Anti-CD3 epsilon antibody [PPT3] (PE/Cy5®) (ab25535)

Overview

  • Product name
    Anti-CD3 epsilon antibody [PPT3] (PE/Cy5®)
    See all CD3 epsilon primary antibodies
  • Description
    Mouse monoclonal [PPT3] to CD3 epsilon (PE/Cy5®)
  • Conjugation
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applications
    Suitable for: Cellular Activation, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    Porcine CD3e is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. ab25535 PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab25535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Cellular Activation Use at an assay dependent concentration.
Flow Cyt Use 0.2µg for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

References

This product has been referenced in:
  • Walsh MC  et al. Effects of feeding Bt MON810 maize to pigs for 110 days on peripheral immune response and digestive fate of the cry1Ab gene and truncated Bt toxin. PLoS One 7:e36141 (2012). Flow Cyt ; Pig . Read more (PubMed: 22574138) »
  • Yang H  et al. Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes. Immunology 88:577-85 (1996). Read more (PubMed: 8881760) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry.

We do not have any information available about details of the immunogen of this clone of antibody.

We did not perform epitope mapping.

But I can confirm that the epitope is extracellular, becaus...

Read More

Thank you for contacting us. The functional effects of the CD3 epsilon antibody ab25535 clone PPT3 are described in the reference PMID:8881760 (see attached). I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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