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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD3 epsilon antibody, prediluted
See all CD3 epsilon products (44) ...
Description
Rabbit polyclonal to CD3 epsilon, prediluted
Specificity
This antibody recognises CD3 epsilon.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human, Monkey
Predicted to work with
Mouse, Rat, Horse, Cat, Dog, Baboon
Immunogen
Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon.
KAKAKPVTRG AGA
Positive control
Human tonsil for Immunohistochemistry.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 19 kDa.
Target
Function
The CD3 complex mediates signal transduction.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP07766
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 3E antibody
- CD3 TCR complex antibody
- CD3E antibody
see all
Database links
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
- SwissProt: Q5R1Q1 Cat
- SwissProt: P27597 Dog
- SwissProt: P07766 Human
- SwissProt: P22646 Mouse
see all
Anti-CD3 epsilon antibody, prediluted images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD3 epsilon antibody, prediluted (ab27434)
Ab27434, prediluted, staining CD3 epsilon in paraffin embedded, formalin fixed human tonsil by Immunohistochemistry.
Western blot - CD3 epsilon antibody, prediluted (ab27434)
Anti-CD3 epsilon antibody, prediluted (ab27434) at 1/25 dilution + Jurkat cell lysate
Predicted band size : 19 kDa
Observed band size : 19 kDa
This dilution in this image refers to the dilution used for a non-prediluted version of this product.
References for Anti-CD3 epsilon antibody, prediluted (ab27434)
This product has been referenced in:
- Song Yet al. Inflammation, Endothelial Injury, and Persistent Pulmonary Hypertension in Heterozygous BMPR2-Mutant Mice. Am J Physiol Heart Circ Physiol : (2008).Read more (PubMed: 18552156) »
See 1 publication for this product
Publishing research using ab27434? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"