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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD3 epsilon antibody, prediluted (ab27434)
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Product Name
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CD3 epsilon antibody, prediluted
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See all CD3 epsilon antibodies (40)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD3 epsilon, prediluted
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Immunogen
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Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon.
BLAST 'KAKAKPVTRGAGA' with  or 
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Reacts with
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Hu, Bb, Cat, Dog, Hrs, Mk
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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This antibody recognises CD3 epsilon.
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: use neat for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human tonsil for Immunohistochemistry.
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains
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CD3 epsilon antibody, prediluted (ab27434) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Prediluted
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD3 epsilon antibody, prediluted is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD3 epsilon antibody, prediluted images, references, products related to ab27434 and other tools.
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CD3 epsilon antibody, prediluted images:
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 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD3 epsilon antibody, prediluted (ab27434)
Ab27434, prediluted, staining CD3 epsilon in paraffin embedded, formalin fixed human tonsil by Immunohistochemistry.
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References for CD3 epsilon antibody, prediluted (ab27434)
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This product has been used in:
Song Y et al. Inflammation, Endothelial Injury, and Persistent Pulmonary Hypertension in Heterozygous BMPR2-Mutant Mice. Am J Physiol Heart Circ Physiol : (2008). PubMed: 18552156
If you publish research using ab27434 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD3 epsilon
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CD3 epsilon antibody, prediluted - more information
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Contact Abcam with a Technical Enquiry about CD3 epsilon antibody, prediluted |
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