Human spleen and thymus tissues; CD3 zeta transfected 293T cell lysate; Jurkat cell lysate.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab190728 as a replacement.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
The ITAM domains mediate interaction with SHB.
Phosphorylated on Tyr residues after T-cell receptor triggering.