The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/200 for 10 minutes at RT. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
The ITAM domains mediate interaction with SHB.
Phosphorylated on Tyr residues after T-cell receptor triggering.