Overview

  • Product nameAnti-CD3 zeta antibody [H146-968]
    See all CD3 zeta primary antibodies
  • Description
    Armenian Hamster monoclonal [H146-968] to CD3 zeta
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Pig
  • Immunogen

    Synthetic peptide:

    DTYDALHMQTLAPR

    , corresponding to amino acids 151-164 of Mouse CD3 zeta.

  • Positive control
    • Mouse splenocyte lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE.
  • ClonalityMonoclonal
  • Clone numberH146-968
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa.
IP Use at an assay dependent concentration.
ICC Use a concentration of 4 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • Sequence similaritiesBelongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • DomainThe ITAM domains mediate interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after T-cell receptor triggering.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

Anti-CD3 zeta antibody [H146-968] images

  • Anti-CD3 zeta antibody [H146-968] (ab119827) at 1 µg/ml + Mouse splenocyte lysate

    Performed under reducing conditions.

    Predicted band size : 19 kDa

References for Anti-CD3 zeta antibody [H146-968] (ab119827)

This product has been referenced in:
  • Iida T  et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). Read more (PubMed: 11046036) »
  • Kosugi A  et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). Read more (PubMed: 10464160) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"