Anti-CD3 zeta antibody [H146-968] (FITC) (ab91493)

Overview

  • Product name
    Anti-CD3 zeta antibody [H146-968] (FITC)
    See all CD3 zeta primary antibodies
  • Description
    Armenian Hamster monoclonal [H146-968] to CD3 zeta (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    DTYDALHMQALPPR

    , corresponding to amino acids 151-164 of Mouse CD3 zeta

  • General notes

    Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Size exclusion
  • Clonality
    Monoclonal
  • Clone number
    H146-968
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at a concentration of 2 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • Involvement in disease
      Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • Sequence similarities
      Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • Domain
      The ITAM domains mediate interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues after T-cell receptor triggering.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    References for Anti-CD3 zeta antibody [H146-968] (FITC) (ab91493)

    ab91493 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Flow Cytometry
    Sample
    Chicken Cell (PBMC)
    Permeabilization
    Yes - BD Fix/Perm
    Gating Strategy
    Lymphocytes/Singlets/CD3/CD3zeta
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS/5% FCS
    Fixation
    BD Fix/Perm
    Username

    Stig Henrik Andersen

    Verified customer

    Submitted May 19 2017

    We do not have any more of ab11505 in stock. Are you able to use the PE-conjugated version of the same clone, ab33395, or the unconjugated version, ab11218? Alternatively, we have a FITC-conjugated hamster monoclonal antibody that is raised against mou...

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    Thank you for your reply.

    Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeab...

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    Thank you for your enquiry.

    I have done some literature searching and am not able to find any information to indicate that the membrane and cytoplasmic forms of CD3 Zeta are different, apart from the localization. Therefore, I would suggest ...

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    Thank you for your response.

    ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse an...

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    Thank you for your enquiry.

    Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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