The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: use neat for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
Staining pattern: Cell membrane
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Sequence similaritiesBelongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
DomainThe ITAM domains mediate interaction with SHB.
Post-translational modificationsPhosphorylated on Tyr residues after T-cell receptor triggering.