Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
The ITAM domains mediate interaction with SHB.
Phosphorylated on Tyr residues after T-cell receptor triggering.