Anti-CD3 zeta (phospho Y142) antibody (ab59175)

Overview

  • Product name
    Anti-CD3 zeta (phospho Y142) antibody
    See all CD3 zeta primary antibodies
  • Description
    Rabbit polyclonal to CD3 zeta (phospho Y142)
  • Specificity
    Detects endogenous levels of CD3 zeta only when phosphorylated at tyrosine 142. Recognises human phosphorylated at tyrosine 142 and mouse phosphorylated at tyrosine 143.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide (Human) around the phosphorylation site of tyrosine 142 (GLYPQG)

  • Positive control
    • Jurkat cell extract treated with UV

Properties

Applications

Our Abpromise guarantee covers the use of ab59175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA 1/5000.

Target

  • Function
    Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • Involvement in disease
    Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • Sequence similarities
    Belongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • Domain
    The ITAM domains mediate interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after T-cell receptor triggering.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

Images

  • All lanes : Anti-CD3 zeta (phospho Y142) antibody (ab59175) at 1/500 dilution

    Lane 1 : Jurkat cells treated with UV (15 mins)
    Lane 2 : Jurkat cells treated with UV (15 mins) with phosphopeptide


    Predicted band size : 23 kDa
    Observed band size : 23 kDa

References

ab59175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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