Anti-CD3 zeta (phospho Y142) antibody (ab59175)

Overview

  • Product nameAnti-CD3 zeta (phospho Y142) antibody
    See all CD3 zeta primary antibodies
  • Description
    Rabbit polyclonal to CD3 zeta (phospho Y142)
  • SpecificityDetects endogenous levels of CD3 zeta only when phosphorylated at tyrosine 142. Recognises human phosphorylated at tyrosine 142 and mouse phosphorylated at tyrosine 143.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide (Human) around the phosphorylation site of tyrosine 142 (GLYPQG)

  • Positive control
    • Jurkat cell extract treated with UV

Properties

Applications

Our Abpromise guarantee covers the use of ab59175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA 1/5000.

Target

  • FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • Sequence similaritiesBelongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • DomainThe ITAM domains mediate interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after T-cell receptor triggering.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

Anti-CD3 zeta (phospho Y142) antibody images

  • All lanes : Anti-CD3 zeta (phospho Y142) antibody (ab59175) at 1/500 dilution

    Lane 1 : Jurkat cells treated with UV (15 mins)
    Lane 2 : Jurkat cells treated with UV (15 mins) with phosphopeptide


    Predicted band size : 23 kDa
    Observed band size : 23 kDa

References for Anti-CD3 zeta (phospho Y142) antibody (ab59175)

ab59175 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59175.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"