The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa).
1/250 - 1/500.
Is unsuitable for Flow Cyt or IHC-P.
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
The ITAM domains mediate interaction with SHB.
Phosphorylated on Tyr residues after T-cell receptor triggering.
Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235)
All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/1000 dilution
Lane 1 : Untreated Jurkat cells whole cell lysates Lane 2 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates Lane 3 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lee J et al. Induction of Immunosuppressive CD8+CD25+FOXP3+ Regulatory T Cells by Suboptimal Stimulation with Staphylococcal Enterotoxin C1. J Immunol200:669-680 (2018).
Read more (PubMed: 29237775) »
Li L et al. Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling. Proc Natl Acad Sci U S A114:E5891-E5899 (2017).
Read more (PubMed: 28659468) »