Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235)

Overview

  • Product name
    Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]
    See all CD3 zeta primary antibodies
  • Description
    Rabbit monoclonal [EP265(2)Y] to CD3 zeta (phospho Y142)
  • Specificity
    This antibody detects CD3 zeta phosphorylated on tyrosine 142.
  • Tested applications
    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 142 of human CD3 zeta.

  • Positive control
    • Jurkat cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP265(2)Y
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab68235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa).
IP 1/50.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • Involvement in disease
      Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • Sequence similarities
      Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • Domain
      The ITAM domains mediate interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues after T-cell receptor triggering.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    Images

    • All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cells whole cell lysates
      Lane 2 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates
      Lane 3 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 19 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/2000 dilution

      Lane 1 : Jurkat cell lysates, membrane un-treated
      Lane 2 : Jurkat cell lysates, membrane treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 19 kDa

    References

    This product has been referenced in:
    • Pageon SV  et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. Proc Natl Acad Sci U S A 113:E5454-63 (2016). ICC/IF . Read more (PubMed: 27573839) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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