The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa).
1/250 - 1/500.
Application notesIs unsuitable for Flow Cyt or IHC-P.
FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Sequence similaritiesBelongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.
DomainThe ITAM domains mediate interaction with SHB.
Post-translational modificationsPhosphorylated on Tyr residues after T-cell receptor triggering.
Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235)
All lanes : Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235) at 1/1000 dilution
Lane 1 : Untreated Jurkat cells whole cell lysates Lane 2 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates Lane 3 : Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution