Overview

  • Product nameAnti-CD30 antibody
    See all CD30 primary antibodies
  • Description
    Goat polyclonal to CD30
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Recombinant mouse extracellular domain, expressed in mouse NSO cells. The extracellular domain is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.

  • General notesEndotoxin: <10 ng/mg antibody determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml; detects mouse CD30 at approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
    • Sequence similaritiesContains 6 TNFR-Cys repeats.
    • Post-translational
      modifications
      Phosphorylated on serine and tyrosine residues.
    • Cellular localizationCytoplasm and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    References for Anti-CD30 antibody (ab10957)

    ab10957 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"