Anti-CD30 antibody [Ber-H2], prediluted (ab871)

Overview

  • Product nameAnti-CD30 antibody [Ber-H2], prediluted
    See all CD30 primary antibodies
  • Description
    Mouse monoclonal [Ber-H2] to CD30, prediluted
  • SpecificityThis antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were injected with L428 cell line cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
    Although not required, microwave treatment will considerably enhance the signal.
  • Target

    • FunctionReceptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
    • Sequence similaritiesContains 6 TNFR-Cys repeats.
    • Post-translational
      modifications
      Phosphorylated on serine and tyrosine residues.
    • Cellular localizationCytoplasm and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    Anti-CD30 antibody [Ber-H2], prediluted images

    • IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-CD30 antibody [Ber-H2], prediluted (ab871)

    ab871 has not yet been referenced specifically in any publications.

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