Immunoprecipitation analysis of Jurkat cell lysate (in NETN buffer, 1 mg per IP reaction; 20% of IP loaded) labeling CD30 using ab190496 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated CD30, ab190496 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-CD30 antibody (ab190496)
All lanes : Anti-CD30 antibody - N-terminal (ab190496) at 1 µg/ml
Lane 1 : HeLa cell lysate in NETN buffer Lane 2 : 293T cell lysate in NETN buffer Lane 3 : Jurkat cell lysate in NETN buffer