Overview

  • Product name
    Anti-CD30 antibody - N-terminal
    See all CD30 primary antibodies
  • Description
    Rabbit polyclonal to CD30 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human CD30 aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    MRVLLAALGL LFLGALRAFP QDRPFEDTCH GNPSHYYDKA VRRCCYRCPM


    Database link: P28908

  • Positive control
    • Jurkat and 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/500 - 1/2500.

Target

  • Function
    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
  • Sequence similarities
    Contains 6 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localization
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 30 antibody
    • CD30 antibody
    • CD30 antigen antibody
    • CD30L receptor antibody
    • Cytokine receptor CD30 antibody
    • D1S166E antibody
    • KI 1 antibody
    • KI 1 antigen antibody
    • Ki-1 antigen antibody
    • KI1 antibody
    • Lymphocyte activation antigen CD30 antibody
    • TNFRSF 8 antibody
    • Tnfrsf8 antibody
    • TNR8_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 8 antibody
    see all

Images

  • Immunoprecipitation analysis of Jurkat cell lysate (in NETN buffer, 1 mg per IP reaction; 20% of IP loaded) labeling CD30 using ab190496 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated CD30, ab190496 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

  • All lanes : Anti-CD30 antibody - N-terminal (ab190496) at 1 µg/ml

    Lane 1 : HeLa cell lysate in NETN buffer
    Lane 2 : 293T cell lysate in NETN buffer
    Lane 3 : Jurkat cell lysate in NETN buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 30 seconds

References

ab190496 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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