Overview

  • Product nameAnti-CD300 antibody [MEM-260]
    See all CD300 primary antibodies
  • Description
    Mouse monoclonal [MEM-260] to CD300
  • Specificityab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HPB human lymphoid leukemia cell line.

  • Positive control
    • Positive material: HPB cell line. Negative material: Jurkat human cell line, Raji human cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from hybridoma culture supernatant by protein-A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberMEM-260
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 35 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation.
  • Tissue specificityHighly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.
  • Sequence similaritiesBelongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD300 antigen like family member antibody
    • CD300 antigen-like family member F antibody
    • CD300 molecule like family member f antibody
    • CD300f antibody
    • CD300LF antibody
    • CLM antibody
    • CLM-1 antibody
    • CLM1 antibody
    • CLM1_HUMAN antibody
    • CMRF35-like molecule 1 antibody
    • IGSF antibody
    • IgSF13 antibody
    • Immune receptor expressed on myeloid cells 1 antibody
    • immune receptor expressed on myeloid cells antibody
    • immunoglobin superfamily member antibody
    • Immunoglobulin superfamily member 13 antibody
    • Immunoglobulin superfamily member antibody
    • Inhibitory receptor IREM1 antibody
    • IREM antibody
    • IREM-1 antibody
    • IREM1 antibody
    • Nepmucin antibody
    • NK inhibitory receptor antibody
    • NKIR antibody
    • TREM antibody
    • triggering receptors expressed on myeloid cells antibody
    • UNQ3105/PRO10111 antibody
    see all

References for Anti-CD300 antibody [MEM-260] (ab53317)

ab53317 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab53317.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"