Specificityab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c).
The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 35 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionActs as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation.
Tissue specificityHighly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.
Sequence similaritiesBelongs to the CD300 family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational modificationsPhosphorylated on tyrosine.