Overview

  • Product nameAnti-CD300C antibody
    See all CD300C primary antibodies
  • Description
    Rabbit polyclonal to CD300C
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    internal sequence amino acids 115-183 (PWLRDFHDPI VEVEVSVFPA GTTTASSPQS SMGTSGPPTK LPVHTWPSVT RKDSPEPSPH PGSLFSNVR) of Human CD300C.

  • Positive control
    • Human spleen tissue; Transfected HEK293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificityPresent on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.
  • Sequence similaritiesBelongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD300 antigen-like family member C antibody
    • CD300c antibody
    • CD300c antigen antibody
    • CLM-6 antibody
    • CLM6_HUMAN antibody
    • CMRF-35 antibody
    • CMRF-35A antibody
    • CMRF35 antibody
    • CMRF35 antigen antibody
    • CMRF35 leukocyte immunoglobulin-like receptor antibody
    • CMRF35-A1 antibody
    • CMRF35-like molecule 6 antibody
    • CMRF35A antibody
    • CMRF35A leukocyte immunoglobulin-like receptor antibody
    • CMRF35A1 antibody
    • IgSF16 antibody
    • Immunoglobulin superfamily member 16 antibody
    • LIR antibody
    see all

Anti-CD300C antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei. Recommended concentration of ab121435 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121435 at a 1/250 dilution staining CD300C in paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • All lanes : Anti-CD300C antibody (ab121435) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : CD300C transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)

    Developed using the ECL technique

References for Anti-CD300C antibody (ab121435)

ab121435 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"